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TITLE PAGE

PHYTOCHEMICAL AND ANTIMALARIAL PROPERTIES OF MANGIFERA INDICA

BY

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--/H2013/01430
DEPARTMENT OF ----
SCHOOL OF ---
INSTITUTE OF ---

DECEMBER,2018

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APPROVAL PAGE

This is to certify that the research work, "phytochemical and antimalarial properties of mangifera indica" by ---, Reg. No. --/H2007/01430 submitted in partial fulfillment of the requirement award of a Higher National Diploma on --- has been approved.

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Supervisor                                                  Head of Department.
Signature……………….                           Signature……………….        

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External Invigilator

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DEDICATION
This project is dedicated to Almighty God for his protection, kindness, strength over my life throughout the period and also to my --- for his financial support and moral care towards me.Also to my mentor --- for her academic advice she often gives to me. May Almighty God shield them from the peril of this world and bless their entire endeavour Amen.

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ACKNOWLEDGEMENT

The successful completion of this project work could not have been a reality without the encouragement of my --- and other people. My immensely appreciation goes to my humble and able supervisor mr. --- for his kindness in supervising this project.
My warmest gratitude goes to my parents for their moral, spiritual and financial support throughout my study in this institution.
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The menace of current cases of parasite resistance to anti-malarial drugs, non- availability and accessibility, and the high costs of pharmaceutical products contribute to the high rate of medicinal plants consumption in the treatment of malaria in Nigeria. Different ethnobotanical surveys on medicinal plants with anti-malarial properties have been conducted across different geographical regions in Nigeria in order to identify and select the most commonly used antimalarial plants as candidates in the proposed national herbal pharmacopoeia. The medicinal plants used against malaria in the western, central, eastern and northern geographical regions in Nigeria was discovered to be mangifera indica. Mangifera indica was discovered with 80% appearance each across the 4 regions and was therefore ranked as the most used for treatment of malaria in Nigeria. This research was aimed to determine phytochemical composition and anti-malarial activity of leaves extracts of Mangifera indica. Powdered leaves of Mangifera indica were extracted with water, ethanol and chloroform solvents via percolation method. The extracts were tested for antibacterial activity against clinical isolates of Salmonella Typhi, Salmonella Paratyphi A and Salmonella Paratyphi B using agarwell diffusion method. The extracts were further subjected to phytochemical tests for the presence of secondary metabolites using standard procedures. Results of the sensitivity test showed that highest zone of inhibition was observed in ethanolic extract with 13mm for S. Paratyphi A, 11mm for S. Paratyphi B, and 10mm for S. Typhi, followed by aqueous extract with 11mm for S. Paratyphi B and 10mm for S. Typhi. S. Paratyphi A, was resistant to both aqueous and chloroform extract of M. indica while S. Paratyphi B was only resistant to chloroform extract. Results of phytochemical screening indicated the presence of alkaloids, flavonoids, steroids, tannins and phenols. 

TABLE OF CONTENTS
COVER PAGE
TITLE PAGE
APPROVAL PAGE
DEDICATION
ACKNOWELDGEMENT
ABSTRACT
CHAPTER ONE

1.  INTRODUCTION
2. BACKGROUND OF THE STUDY
3. PROBLEM STATEMENT
4. AIM AND OBJECTIVE OF THE STUDY
5. SIGNIFICANCE OF THE STUDY
6. SCOPE OF THE PROJECT
7. PROJECT ORGANISATION

CHAPTER TWO
LITERATURE REVIEW

1. REVIEW OF THE STUDY
2. OVERVIEW OF MANGO (MANGIFERA INDICA L.)
3. PLANT DESCRIPTION
4. ETHNOMEDICINAL USE OF MANGO
5. NUTRIENT AND PHYTOCHEMICALS OF MANGO
6. PHARMACOLOGICAL USE OF MANGO

CHAPTER THREE

MATERIALS AND METHODS

1. COLLECTION AND IDENTIFICATION OF   PLANT MATERIALS
2. EXTRACTION
3. PHYTOCHEMICAL SCREENING

CHAPTER FOUR

1. RESULT AND DISCUSSION

CHAPTER FIVE

1. CONCLUSION
2. REFERENCES

CHAPTER ONE
1.0                                        INTRODUCTION
1.1                               BACKGROUND OF STUDY
Malaria remains one of the major health challenges in developing countries despite the efforts of different organizations including the World Health Organization (WHO), West African Health Organization (WAHO), Centers for Disease Control and Prevention (CDC), the African Union's Scientific, Technical and Research Commission (AU/STRC) among others to control and eradicate it (WHO, 2018).

It was reported that 219 million cases of malaria occurred worldwide in 2017 and 92% of these cases were from African region with 435,000 mortalities. The setback to malaria fight has been attributed to non- availability of effective vaccine, resistance to pyrethroid- treated mosquito nets, high costs of anti-malarial drugs and the recent widespread chloroquine-resistant Plasmodium falciparum (WHO, 2018).

In malaria chemotherapy, medicinal plants have always played a leading role in drug discovery and such drugs are used in natural form or synthesized or act as structural models for semi-synthetic anti-malarial drugs. Quinine was first time isolated from Cinchona bark against malaria in the early 18th century and became a skeleton from which chloroquine (resochin), mefloquine and other similar antimalarial drugs were later synthesized (Achan et al., 2011).
Historically (from Cinchona to Artemisia), the plant kingdom remains the source for antimalarial drug discovery. Similar history has shown many current therapeutic drugs (e.g. digoxin, reserpine, morphine, etc.), at conventional health care levels for the management of other diseases from medicinal plants. According to the World Health Organization, 60% of the world’s population depends on traditional medicine and 80% of the people in developing countries depend entirely on traditional medicine practices due to their accessibility, folklore and affordability for their primary health care needs (Chikezie and Ojiako, 2015).
The high acceptability of medicinal plants therefore requires the needs for their national standards which guarantee the consistence, definite identification, reproducible safety, efficacy and qualities as a valuable scientific reference for drug authorities, manufacturers, general public and researchers (WHO, 2011). These plants are normally selected based on their frequent used across the country.
Mango (Mangifera indica)‘king of fruit’ belongs to use pharmacological potential as panacea. Mangifera indica leaves are used for the treatments of various ailments in folklore medicine. Mango extracts from leaves, fruit, seed kernel, fruit pulp, roots, bark and stem bark have been used extensively for medicinal purposes in many countries5. The ethnomedical use of mango stem bark aqueous extract in Cuba has been documented widely5.   It has been extensively used in cancer, diabetes, asthma, infertility, lupus, prostatisis, prostatic hyperplasia, gastric disorders, arthralgies, mouth sores and tooth pain.
This research was aimed to determine phytochemical composition and antimalarial activity of extracts of Mangifera indica.

1.2                         PROBLEM STATEMENT
Clinical drug are said to be traceable to the major cause of some chronic disease in most cases when it is wrongly taken and it is also costly. Because of these problems there is an urgent need to substantiate scientifically the roles of contemporary and native medicinal practices. This exercise will open up a new class of drugs which could be cheap, easily affordable, and with less or no side effects. mangifera indica is known to a wonder herbal plant and most of its parts are used to cure various ailments such as malarial.

1.3                          AIM OF THE PROJECT
The aim of this research was to determine the phytochemical composition of the leaves extract of M. indica used as traditional medicine and treatment of malarial.

1.4                   SIGNIFICANCE OF THE STUDY
This study has exposed us to the used us to different use of mango leave most especially in treatment of malarial. From this study i shall be exposed to reasons while herbal medicines are chose over clinical drugs.

1.5                              SCOPE OF THE STUDY
This study analyses the phytochemical and anti-malarial properties of mangifera indica. From the study, extracts were further subjected to phytochemical tests for the presence of secondary metabolites using standard procedures. Results of phytochemical screening indicated the presence of alkaloids, flavonoids, steroids, tannins, phenols and that mangifera indica is a good anti-malarial herb.

1.6                       PROJECT ORGANISATION
The work is organized as follows: chapter one discuses the introductory part of the work,   chapter two presents the literature review of the study,  chapter three describes the methods applied, chapter four discusses the results of the work, chapter five summarizes the research outcomes and the recommendations.

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